3B BlackBio Biotech High Retrotranscriptase is a thermostable, new recombinant reverse transcriptase expressed in E. coli.

The enzyme is an RNase H minus which exhibits high affinity for RNA and works in a higher range of temperature than most commercially retrotranscriptases. Due to its thermostability, 3B High Retrotranscriptase can transcribe GC-rich templates with large amounts of secondary structure without the help of reaction additives.

3B High Retrotranscriptase will accept three different types of primer for cDNA synthesis: Oligo (dT) primer; random hexamer primers; or a gene-specific primer.

The enzyme can be used at a temperature up to 65°C, and it is recommended for RT-PCR or qRT-PCR.

Product applications:

·         Synthesis of first strand cDNA for use in subsequent amplification reactions: RT-PCR or qRT-PCR.

·         RT-PCR amplification of difficult RNA templates, such as GC-rich templates with large amounts of secondary structure

·         Incorporation of Cy3-, Cy5-, Biotin-, or aminoallyl-modified nucleotides during cDNA synthesis (e.g., for use in microarray hybridization).